Concept of PhenoDot
A new platform for immunoassays requiring minimal equipment, virtually no barrier to entry and minimal training with results visible to the naked-eye. Moreover results are so obvious they can simply be recorded by mobile phone camera or macro-lens fitted digital camera.
Identify, Quantify, & Isolate
Cells are encapsulated forming visible dots for fast and simple quantification, and the system boasts single cell resolution while maintaining cell viability.
Key Features of PhenoDot
Setting PhenoDot apart from other immunoassays.
Maintains Viability.
By design our PhenoDot reagent is cell compatible, simultaneous identification of target cells, isolation, and recovery in a single step assay. With correct dilution is possible to tag cells and simply pick individual positively tagged cells by eye with capillary pipette in 1 step.
Compatible with any platform.
With little or no specific equipment requirements an assay using PhenoDot can be adapted to any cell platform from petri-dish to 96 well plates and more, offering complete flexibility according to the experiment design.
Cost Saving.
Without the need for systems like flow cytometers and plate readers, PhenoDot assays offer a virtual zero barrier to entry solution for immunoassays. No expensive equipment, training, maintenance, or workspace required.
Time Saving.
The potential to carry out all the phenotyping and isolation down to a single cell in a single step means PhenoDot offers huge time savings. Cells can be detected by multiple markers and positively selected directly for use. For example PhenoDot can reduce much of the time expended in selecting and depleting cells to produce a monoclonal antibody or for cell cloning.
Improved Accuracy.
By direct detection of cells by multiple markers and marker types PhenoDot removes much of the assumptions made when studying cells by multiple techniques such as FACs and ELISpot. Moreover, cells undergo less external treatment so remain closer to native state than might otherwise be true.
Flexibility.
Potential for huge adaptability is a super strength of PhenoDot for example it’s use is not limited to cells only but also microorganisms. Assays can be designed to develop single visible dots in response to a multitude of markers giving a single binary response assay or by use of different enzyme substrates a multiple response data rich assay. Various platform compatibility can allow small or large sample size analysis as well as alternative analysis during or after phenotyping by PhenoDot.
PhenoDot Simplified
At the very heart of it PhenoDot is a simplification on all forms of immunoassay. Cells are prepared and then incubated in a label of choice for the required analysis. Once labelled, our reagent applied is applied. Within minutes we can start to see colour forming localised at the labelled cell site and in less than 1 hour results are visible to the eye and ready to be recorded.
4 SIMPLE STEPS TO IMMUNOASSAY RESULTS.
Prepare - Label - Develop - Record